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Ribosome Profiling Data Analysis: from BAM to Data Representation and Interpretation

Bioconductor version: Release (3.19)

Starting with a BAM file, this package provides the necessary functions for quality assessment, read start position recalibration, the counting of reads on CDS, 3'UTR, and 5'UTR, plotting of count data: pairs, log fold-change, codon frequency and coverage assessment, principal component analysis on codon coverage.

Author: Alexandra Popa

Maintainer: A. Popa <alexandra.mariela.popa at>

Citation (from within R, enter citation("RiboProfiling")):


To install this package, start R (version "4.4") and enter:

if (!require("BiocManager", quietly = TRUE))


For older versions of R, please refer to the appropriate Bioconductor release.


To view documentation for the version of this package installed in your system, start R and enter:

Analysing Ribo-Seq data with the "RiboProfiling" package PDF R Script
Reference Manual PDF


biocViews Alignment, Coverage, PrincipalComponent, QualityControl, RiboSeq, Sequencing, Software
Version 1.34.0
In Bioconductor since BioC 3.2 (R-3.2) (8.5 years)
License GPL-3
Depends R (>= 3.5.0), Biostrings
Imports BiocGenerics, GenomeInfoDb, GenomicRanges, IRanges, reshape2, GenomicFeatures, grid, plyr, S4Vectors, GenomicAlignments, ggplot2, ggbio, Rsamtools, rtracklayer, data.table, sqldf
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Suggests knitr, BiocStyle, TxDb.Hsapiens.UCSC.hg19.knownGene, BSgenome.Hsapiens.UCSC.hg19, testthat, SummarizedExperiment
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Follow Installation instructions to use this package in your R session.

Source Package RiboProfiling_1.34.0.tar.gz
Windows Binary
macOS Binary (x86_64) RiboProfiling_1.34.0.tgz
macOS Binary (arm64) RiboProfiling_1.34.0.tgz
Source Repository git clone
Source Repository (Developer Access) git clone
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