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\title{FlowSorted.DLPFC.450k User's Guide\\
  A Public Illumina 450k Dataset}
\author{Andrew E. Jaffe}
\date{Modified: April 8, 2014.  Compiled: \today}


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\section{Introduction}

The \Rpackage{FlowSorted.DLPFC.450k} package contains publicly available Illumina
HumanMethylation450 (``450k'') DNA methylation microarray data from a recent publication by
Guintivano et al. 2013 \cite{guintivano2013}, consisting of 58 samples, formatted as an
\Rcode{RGChannelSet} object for easy integration and normalization using existing Bioconductor
packages. For example, this dataset may be useful ``example'' data for other packages exploring,
normalizing, or analyzing DNA methylation data.

Researchers may find this package useful as these samples represent two different cellular
populations of brain tissue generated on the same 29 individuals using flow sorting, a experimental
procedure that can separate heterogeneous biological samples like brain into more pure cellular
populations, like neurons and non-neurons using NeuN labeling \cite{akbar2008}. This data can be
directly integrated with the \Rpackage{minfi} Bioconductor package to estimate cellular composition
in users' frontal cortex Illumina 450k samples using a modified version of the algorithm described
in Houseman et al. 2012 \cite{houseman2012} and Guintivano et al 2013 \cite{guintivano2013}.

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