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Targeted scRNA-seq primer design for TAP-seq

Bioconductor version: Release (3.19)

Design primers for targeted single-cell RNA-seq used by TAP-seq. Create sequence templates for target gene panels and design gene-specific primers using Primer3. Potential off-targets can be estimated with BLAST. Requires working installations of Primer3 and BLASTn.

Author: Andreas R. Gschwind [aut, cre] , Lars Velten [aut] , Lars M. Steinmetz [aut]

Maintainer: Andreas R. Gschwind <andreas.gschwind at>

Citation (from within R, enter citation("TAPseq")):


To install this package, start R (version "4.4") and enter:

if (!require("BiocManager", quietly = TRUE))


For older versions of R, please refer to the appropriate Bioconductor release.


To view documentation for the version of this package installed in your system, start R and enter:

Select target genes for TAP-seq HTML R Script
TAP-seq primer design workflow HTML R Script
Reference Manual PDF


biocViews CRISPR, PooledScreens, Sequencing, SingleCell, Software, Technology
Version 1.16.0
In Bioconductor since BioC 3.11 (R-4.0) (4 years)
License MIT + file LICENSE
Depends R (>= 4.0.0)
Imports methods, GenomicAlignments, GenomicRanges, IRanges, BiocGenerics, S4Vectors(>= 0.20.1), GenomeInfoDb, BSgenome, GenomicFeatures, Biostrings, dplyr, tidyr, BiocParallel
System Requirements Primer3 (>= 2.5.0), BLAST+ (>=2.6.0)
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Suggests testthat, BSgenome.Hsapiens.UCSC.hg38, knitr, rmarkdown, ggplot2, Seurat, glmnet, cowplot, Matrix, rtracklayer, BiocStyle
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Follow Installation instructions to use this package in your R session.

Source Package TAPseq_1.16.0.tar.gz
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macOS Binary (x86_64) TAPseq_1.16.0.tgz
macOS Binary (arm64) TAPseq_1.16.0.tgz
Source Repository git clone
Source Repository (Developer Access) git clone
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